Integration of proteomic and transcriptomic data of the venom and venom gland of Tityus jaimei.

This work presents a comprehensive transcriptomic and proteomic analysis of the venom gland and venom of titus jaimeia species of scorpion recently described and of medical relevance in Panama and Costa Rica. High-throughput RNA sequencing (RNA-seq) and tandem mass spectrometry (MS/MS) enabled the identification of a diverse repertoire of venom proteins. In particular, this is the first report of proteins belonging to the nucleotide pyrophosphatase/phosphodiesterase and knot families in the venom proteome of a scorpion of the genus Ticio. Additionally, several families of known poison proteins were identified, including hyaluronidases, voltage-gated sodium and potassium channel toxins, lipolysis-activating peptides (LVPs), cysteine-rich secretory proteins (CRISPs), metalloproteinases, peptidylglycine alpha-hydroxylating monooxygenases. (PHM), serine proteases, alpha-amylases, insulin-like growth factor single domain binding proteins, non-disulfide bonded peptides (NDBP), chitinases, cyclotide trypsin inhibitors and calcine-like peptides. The identification of 16 different families in the venom of titus jaimei offers novel insights into its composition and the diversity of Tityus venoms in Central America. Finally, the use of AI for protein domain search for protein annotation.